FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53139226
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTCG5836441.0983298853468433Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT645000.042.35594610
CGTGTAG654000.041.74402614
AGCGTCG660500.041.4198769
ATCGGAA664850.041.1587031
GTCGTGT665000.041.12056712
TCGTGTA664450.041.07057613
GTAGATC666000.040.69200542
CGTCGTG678250.040.3458611
AGAGCGT682050.040.1374137
GATCTCG675150.040.0038145
GTGTAGG684900.039.95303315
GTCTTCG677800.039.81757730
GTAGGGA689550.039.54758517
GAGCGTC693100.039.5203828
TAGATCT684300.039.51832643
TGTAGGG694900.039.3368516
TCGGAAG707300.038.870532
AAGAGCG706900.038.8226
TCGCCTG707700.038.3112734
GTGTAGA709850.038.27020640
Produced by FastQC (version 0.11.5)