FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52913747
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCG4990590.9431556604751503Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT533250.043.4076210
CGTGTAG544100.042.6780114
ATCGGAA546600.042.6089061
TAGTGTA547550.041.9877838
AGCGTCG554900.041.8025789
GTAGATC551100.041.75702742
AGAGCGT555850.041.7433937
GTCGTGT557250.041.69566312
TCGTGTA561100.041.35772313
ATAGTGT558650.041.11684437
CGTCGTG566050.041.0792411
GAGTGTA565900.040.953726
GTAGGGA566950.040.94145217
GATCTCG567750.040.797145
TAGATCT567050.040.57809443
TGTAGGG573500.040.50980416
GAGCGTC572500.040.4703188
AAGAGCG582950.039.9409986
TCGGAAG591300.039.6349642
GTGTAGA581950.039.55491340
Produced by FastQC (version 0.11.5)