FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50148990
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGC3996730.7969711852621557TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG1167770.23286012340427992TruSeq Adapter, Index 7 (97% over 36bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGT590610.11777106577819413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC478700.042.24172645
TCGTATG589950.034.31029544
TCACCGG606250.033.4798229
CTCGTAT601800.033.406743
ACCGGCT609950.033.26311531
CCGGCTA610550.033.22129432
ACACGTC614650.033.2034412
ACGTCTG614150.033.17553714
CACCGGC622750.032.62686530
GTCACCG622800.032.6094428
GATCGGA172200.032.582291
ATCGGAA630450.032.3692471
CGTCTGA630900.032.26307315
TATGATC572750.032.10173437
TCGGAAG646800.032.020892
CACACGT639800.031.95449811
ATCTCGT578550.031.91174141
CACGTCT641200.031.82158313
TCTCGTA581000.031.77330242
CGGAAGA655800.031.413273
Produced by FastQC (version 0.11.5)