FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49338120
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTCG530050.10743214374605276Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT83150.030.21845210
GTAGATC87500.028.51052942
TACGTGT89400.027.9537937
TAGATCT93550.026.8109943
TCGTGTA95100.026.61111513
GTCGTGT94300.026.5979412
ATCGGAA95850.026.5924661
GTAGGGA99550.025.5342917
GTGTAGG99750.025.30309515
ACGTGTA107100.023.6701438
AGTACGT108450.023.08389535
GATCTCG110250.022.7906345
GTACGTG110500.022.7177936
GTCAGTA112100.022.37383832
TGTAGGG116600.021.87802916
TGTAGAT116050.021.69009441
AGAGCGT116500.021.644957
GTGTAGA120150.020.96819540
GAGCGTC125900.020.3147588
AAGAGCG127950.020.1122826
Produced by FastQC (version 0.11.5)