FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20336096
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCGGAT1936198995.20996065321485No Hit
NGTCGGAT2889111.420680744229374No Hit
TGTCGGGT820120.40328291133165384No Hit
TGTCGGCT661050.32506239152293537No Hit
TGGCGGAT651250.3202433741461488No Hit
TGTCGGAG469300.23077192397203475No Hit
GGTCGGAT392190.1928541250001967No Hit
TGCCGGAT362090.17805285734292364No Hit
TGTGGGAT294960.1450425883119356No Hit
TGTCGGTT283200.1392597674597917No Hit
TGTCGGAA279620.13749935090786353No Hit
TGTTGGAT257210.12647953668196688No Hit
TTTCGGAT255480.12562883259402394No Hit
TGTCTGAT254240.12501907937492032No Hit
TGTAGGAT230900.11354195023469597No Hit
TGTCGGAC208180.10236969770402342Illumina NlaIII expression Adapter 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)