FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19794558
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12600.083.415719
TTTGCGC32700.079.153521
TGCGCGA30300.076.2012941
ACGACCG31350.075.0564041
CGCCGAA30450.074.8599851
CGGTACG29200.074.035461
GACGCGT32200.069.6497041
GCGCGTA31450.067.803581
CCGCTAT37450.067.739591
ACGCCGA36750.067.4291841
GGCGACT39600.067.03281
GTTACGC29800.065.675338
GTGCGAC32350.065.2353441
GTACCGT43450.065.229168
GCGCTAG34450.064.0335161
ATAACGC36650.064.001781
TCGCGAT25900.063.2504238
GTCGGAC27800.062.9517021
CGAACTA41950.062.4012571
ACCGCTT45150.062.3758281
Produced by FastQC (version 0.11.5)