FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19794558
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT28000.095.273566
ACGACGC29700.091.288335
CACGACG30350.088.8557364
GACGCTC41600.064.299887
TACACGA45350.060.1636852
CTACACG49050.056.997171
ACGCTCT48800.055.4071858
ACACGAC53300.051.2761463
CGCTCTT65950.041.5496379
CGTTGAG26704.2018655E-106.90687851
CGTTAGG23807.181552E-76.1987791
GTTCCGA62800.06.11809735
CGCTGAG39801.8189894E-126.11623241
ACGGGAG45500.05.99851041
TAACCCT869700.05.68456846
AACGGGG35657.370545E-95.5867251
CGATGAG27851.9696563E-65.5622071
TCGCGGG18250.00123776675.5609765145
CTAACCC929550.05.4825515
AACCCTA938000.05.44983671
Produced by FastQC (version 0.11.5)