FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18739994
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTTGAC1792467995.64933158463124No Hit
NACTTGAC2642071.4098563745538017No Hit
AACTTGGC438960.23423700135656395No Hit
GACTTGAC424410.22647285799557887No Hit
AGCTTGAC423130.22578982682705234No Hit
CACTTGAC419980.22410892981075659No Hit
AACTTGCC400110.2135059381555832No Hit
TACTTGAC332190.17726259677564465No Hit
AACTGGAC306700.16366067139615945No Hit
AACGTGAC270910.14456247958243743TruSeq Adapter, Index 21 (100% over 8bp)
ACCTTGAC267380.14267880768798538No Hit
AACTTTAC259350.1383938543416823No Hit
AACTTGAA232170.12389011437250193No Hit
AACTTGAG211870.11305766693415163No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)