FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19540981
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT28000.094.758076
ACGACGC29600.091.8413545
CACGACG30150.089.4437264
GACGCTC40300.066.016597
TACACGA43500.062.8284572
CTACACG50100.054.9685551
ACGCTCT50650.052.2402768
ACACGAC54400.049.838733
CGCTCTT65850.040.5120359
AACGGGG36500.08.0479981
CGCTAAG15651.4545488E-46.56953241
TAACCCT877650.05.9470876
CGTTGAG26609.1487163E-75.7977361
CTAACCC953450.05.7860935
AACCCTA957250.05.71546651
ACCCTAA975850.05.61620572
CGTTGGG34402.132947E-85.55054571
CCCTAAC1000150.05.54485943
CCTAACC992400.05.5443334
CGATGGG37203.814421E-95.52759031
Produced by FastQC (version 0.11.5)