FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17290124
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11950.081.285299
TGCGCGA28600.080.0245361
ACGACCG26550.078.442361
CGGTACG27200.077.9206161
TTTGCGC27900.076.2293851
CGCCGAA25700.072.44631
GCGCTAG31800.071.277491
GTGCGAC29150.071.193341
ATAACGC33750.070.8660661
GCGCGTA28500.067.910931
GCCGGAT32350.067.563321
GACGCGT26200.067.1313551
GTTCGCG36300.066.498638
CGAACTA35900.066.007031
GTTACGC28750.065.0514458
GGCGACT35200.064.392731
TAGGCGC33850.064.351971
CGAACGC33150.064.156851
TCGTACG33600.063.2125668
TTACCCG31600.063.1118621
Produced by FastQC (version 0.11.5)