FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005104767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22085321
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA2111694095.6152731490749No Hit
NGTCCAGA3056381.3838965709395847No Hit
GGGCCAGA632430.28635762187925634No Hit
GGTCCAGC473010.21417393027703785No Hit
GGTCGAGA399520.18089843475673276No Hit
GGTGCAGA397810.18012416482422872No Hit
TGTCCAGA397330.17990682589580653No Hit
GGTCCCGA385320.17446882479090978No Hit
GGTCCAGG359120.16260574161453212No Hit
GGTCCGGA323180.14633248934892096No Hit
GGCCCAGA302460.1369506922720299No Hit
GGTTCAGA297820.1348497492972821Illumina DpnII expression Adapter 1 (100% over 8bp)
GGTACAGA268070.12137926362944872No Hit
GGTCCATA235440.10660474439108221No Hit
GGTCCAAA229980.10413251408027983No Hit
GGTCCAGT220880.10001213022894255No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)