FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005104770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22230105
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA2142613896.38343138730114No Hit
NGTCCAGA3191621.4357197143243363No Hit
GGTCCAGC478320.215167674646611No Hit
GGGCCAGA424030.190745837682728No Hit
GGTCCAGG336210.15124085108909743No Hit
GGTCCCGA329030.14801099679916044No Hit
TGTCCAGA300140.13501510676625234No Hit
GGCCCAGA290930.13087207640269805No Hit
GGTCGAGA241160.10848351818401217No Hit
GGTTCAGA226620.10194283832667458Illumina DpnII expression Adapter 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)