FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19920526
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12000.079.737999
TTTGCGC32000.078.871041
TGCGCGA31100.075.94831
CGGTACG33150.074.137241
GCGCTAG32750.069.425761
GACGCGT31150.068.9760361
TTACCCG39900.068.7874761
CGCCGAA30650.068.660831
TAGGCGC38550.067.951511
GTGCGAC34700.067.856891
GGCGACT39500.066.68981
TCGTACG36650.066.060998
CCGCTAT38950.065.931271
ACGACCG29750.065.7912451
CGAACGC35850.065.0644151
GCGCGTA31800.065.020851
TCCGCGA31450.064.306698
GTACCGT44000.063.757448
GTACGCT29850.063.6252568
CGTTGCG16750.063.617287
Produced by FastQC (version 0.11.5)