FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18980845
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA30550.088.155951
TTACGCG11350.076.640059
CGGTACG29350.075.714761
GCGCTAG35000.074.8451461
TTTGCGC30050.071.747191
ACGACCG27500.071.710681
CGAACGC33900.070.978981
GTGCGAC35150.070.75761
TCACGCG27900.070.1503758
GACGCGT29950.070.0212251
ATAACGC35150.068.4548341
TTACCCG35200.068.148551
CGCCGAA28500.066.612631
GCGCGTA27400.066.6013261
GGCGACT35800.065.7731551
ACGCCGA38950.065.176851
GTACCGT40900.063.6270148
GTTACCG27650.062.9196858
TCCGCGA28750.062.7815328
GTTACGC28300.062.4991048
Produced by FastQC (version 0.11.5)