FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18739994
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC48550.0113.493945
CGACGCT49650.0110.693046
CACGACG50450.0109.797414
GACGCTC62700.087.653357
TACACGA65900.084.6271742
CTACACG67550.082.858081
ACGCTCT70300.078.4871448
ACACGAC73750.075.792793
CGCTCTT83600.066.087299
TCTTCCG126150.09.69029410-14
CTTCCGA141050.08.96478810-14
TTCCGAT174350.07.535340310-14
TCCGATC177250.07.16604415-19
CCGATCT188000.06.95681315-19
CGATCTA44800.06.861506515-19
CGTAGAG27901.2405508E-96.58174371
CGGCAAT20501.9396151E-56.09116361
TCGCCGG20251.9470373E-56.0896525145
GCTCTTC213400.06.04093210-14
TAACCCT712400.05.70964776
Produced by FastQC (version 0.11.5)