FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20099962
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12300.086.629659
CGCCGAA31850.078.72871
CGGTACG29000.078.60531
GCGCTAG34500.077.370551
TTTGCGC31700.076.7815551
TGCGCGA29750.076.623661
ACGACCG29700.072.296041
GACGCGT32050.071.124921
CCGCTAT40100.070.783341
GTACGCT27800.070.661378
ATAACGC38300.070.462081
CGAACGC35950.068.931731
TTACCCG38250.068.631741
GTTACGC31050.067.934288
TTCCGCG19500.066.5389567
GTGCGAC35500.066.49131
TAGGCGC39600.065.363551
GGCGACT38600.064.5803761
TCGTACG39150.064.43258
TCGCGAT23200.063.7381948
Produced by FastQC (version 0.11.5)