FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18341241
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT22250.086.667386
ACGACGC24400.079.02925
CACGACG26050.074.30244
GACGCTC34650.055.4434477
TACACGA36400.054.6098142
CTACACG39900.049.2419051
ACGCTCT42000.046.433058
ACACGAC52700.037.694123
CGCTCTT55750.034.850919
CGGTAAG17701.9179588E-67.06760261
CGTAGAG28351.7043931E-96.4890841
TAACCCT656450.06.3609936
AACCCTA706800.06.23629571
CTAACCC702600.06.2010055
ACCGGAG25353.5913217E-76.0958991
ACCCTAA731750.06.0873342
CCCTAAC739450.06.05917263
CCTAACC727650.05.9975424
CGATGGG36604.0381565E-105.83060171
CGTTGAG24759.508605E-65.64904261
Produced by FastQC (version 0.11.5)