FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18299344
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12300.082.502099
TGCGCGA27850.081.7750551
CGCCGAA28850.077.4027561
TTTGCGC29000.077.257371
ACGACCG26750.074.910171
GTGCGAC34000.074.8126141
CGGTACG27100.070.941331
GACGCGT30450.070.4216161
GCGCTAG34050.070.3595661
CCGCTAT38200.068.329251
CGAACGC33350.067.402041
GCGCGTA28500.066.41871
CGAACTA39100.065.432671
GTTACGC30250.065.415298
TCGCGAT22200.064.3213658
GGCGACT36550.063.726261
TCCGCGA27800.063.3582958
GCCGGAT35600.063.142081
TTACCCG35400.062.8721851
AACGCGT26400.062.6619533
Produced by FastQC (version 0.11.5)