FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18299344
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT25750.096.857136
ACGACGC27050.092.733765
CACGACG28250.088.2837754
GACGCTC37950.066.483757
TACACGA42950.059.2639352
CTACACG45150.057.9166151
ACGCTCT46300.054.9634748
ACACGAC49700.050.617253
CGCTCTT60550.041.7887469
TAACCCT689550.06.22452646
CTAACCC729500.06.09206065
CCCTAAC761350.05.960953
CCTAACC755700.05.9481684
TCTTCCG99950.05.87564410-14
ACCCTAA766750.05.87332962
AACCCTA751500.05.796111
CGATGGG32303.1506715E-85.6852351
CGTTGGG31551.14985596E-75.5875681
ATAGGCG28453.149844E-65.42185541
CTTCCGA120050.05.38711110-14
Produced by FastQC (version 0.11.5)