FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18472953
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT22350.090.814556
ACGACGC23100.087.556735
CACGACG25200.081.411244
GACGCTC33800.059.6212047
TACACGA37200.055.7885482
ACGCTCT41200.048.9128048
CTACACG44100.047.6683041
ACACGAC47250.043.726183
CGCTCTT56600.036.5010839
CGCCAAT16403.4733617E-56.74638131
CGGTTAG14704.5985627E-46.52303171
CGCTAAG16150.0014311675.9373721
ACCGGGT24306.7062774E-65.7672771
TAACCCT648150.05.68150236
CTAACCC680800.05.51578765
CGTAAAG21852.9624367E-45.40122181
CGTTGAG24956.2444145E-55.32139441
CCCTAAC718350.05.3082113
TCTTCCG100150.05.298228310-14
CGTTGGG33453.555433E-75.2922281
Produced by FastQC (version 0.11.5)