FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19593186
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1865021195.18722988696172No Hit
NAGTAGAG3321611.69528835177699No Hit
AAGGAGAG851790.43473787264613317No Hit
AAGTCGAG550260.28084253372575546No Hit
AAGTGGAG456250.23286156728160495No Hit
AAGTAGCG377270.19255163504291747No Hit
ACGTAGAG370220.18895344534574418RNA PCR Primer, Index 17 (100% over 8bp)
AGGTAGAG370220.18895344534574418No Hit
AAGTTGAG327320.1670580782523067No Hit
AAGAAGAG320000.16332208554545444No Hit
AAGCAGAG290110.14806678199247433No Hit
AAGTAGGG288810.1474032860199459No Hit
CAGTAGAG252000.12861614236704536No Hit
AAGTAGAA239550.12226189247629253No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)