FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19368060
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA30500.083.453561
TTACGCG12300.078.3836449
CGCCGAA28700.075.6151351
GTGCGAC35200.075.236471
TTTGCGC31000.074.276461
CGGTACG30200.071.8594061
GCGCTAG32850.068.973761
CGAACGC35850.068.742331
ACGACCG28000.068.572671
CGAACTA40300.067.905741
CCGCTAT38650.067.568991
GGCGACT38450.066.963831
TAGGCGC34550.066.218741
GCGCGTA30950.065.36431
TCGCGAT22600.065.112828
TCGTACG34750.064.250258
ACGCCGA40550.063.6773341
GTTCGCG40350.063.5972988
CGATTCG21050.063.430134
CCTTACG40150.062.6627041
Produced by FastQC (version 0.11.5)