FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16114335
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA26700.081.834291
TTACGCG9850.080.953299
TTTGCGC26400.080.534881
CGGTACG27000.078.200271
CGCCGAA24650.074.612781
GTGCGAC27650.074.499441
ACGACCG22550.074.057561
GCGCTAG26250.072.8675461
GACGCGT25700.068.415561
GCCGGAT30450.066.924111
GTTACGC25850.066.741278
ATAACGC27750.066.012541
GGCGACT31950.065.163691
TAGGCGC29700.062.669211
TCGTACG31350.062.2003488
GTACGCT22650.061.768528
CGAACTA34900.061.763561
GCGCGTA24500.061.5570641
GTACCGT37300.061.2180988
CGATCGG29150.060.823111
Produced by FastQC (version 0.11.5)