FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16114335
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC24200.097.656965
CGACGCT24750.095.779716
CACGACG25800.092.442234
GACGCTC35850.066.124067
TACACGA38050.064.2060852
CTACACG40100.060.6159321
ACGCTCT40150.059.5839588
ACACGAC44400.054.696063
CGCTCTT51950.045.4916469
TAACCCT497850.06.1449016
ACGGGAG37602.1827873E-116.0544821
CTAACCC523350.05.91475255
TCTTCCG96600.05.89850610-14
CCCTAAC545750.05.8844273
CCTAACC543300.05.8042194
AACCCTA544300.05.747441
ACCCTAA561050.05.69820362
GTTCCGA42602.5465852E-115.6157185
AGTCGGG27751.1758984E-55.29261351
CGATGGG31052.8212526E-65.20312451
Produced by FastQC (version 0.11.5)