FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19106638
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC53350.0113.869175
CGACGCT53450.0112.702016
CACGACG55000.0110.4539644
TACACGA65950.092.638582
GACGCTC65850.091.5890667
CTACACG69800.088.025871
ACGCTCT73850.082.158018
ACACGAC75800.080.527043
CGCTCTT89300.067.9447259
TCTTCCG127600.010.55525810-14
CTTCCGA148050.09.49876510-14
CGATCTA43550.08.72176615-19
TTCCGAT179700.08.07587810-14
TCCGATC182550.07.53660415-19
CCGATCT195150.07.362009515-19
GCGTCGG26455.0386006E-106.851898145
GCTCTTC221150.06.280328810-14
ACGGGAG42350.06.09586521
GTTCCGA45153.6379788E-125.61961845
TAACCCT763650.05.4677236
Produced by FastQC (version 0.11.5)