FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18514992
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11400.085.84179
CGGTACG28050.081.059881
TGCGCGA25350.079.243761
ACGACCG25900.078.6973951
CGCCGAA30400.077.214261
TTTGCGC27450.076.934281
GTGCGAC32800.075.826881
GCGCTAG31800.072.6579061
GACGCGT27550.071.313211
ATAACGC35250.067.425431
CGAACGC33600.067.0135351
CCGCTAT37600.066.342561
GCGCGTA28600.066.122221
CCTTACG34900.065.993221
TTACCCG32800.065.731591
TCACGCG24600.065.711168
CGAACTA40450.065.306481
CGATCGG35850.064.4496841
GGCGACT36650.062.8421021
GTTACGC26750.062.5975888
Produced by FastQC (version 0.11.5)