FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18628648
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11900.084.057759
TGCGCGA27150.081.249051
GACGCGT28650.075.711921
GTGCGAC31650.074.808171
CGGTACG27950.074.714241
CGCCGAA28950.074.419361
TTTGCGC28600.073.273291
ACGACCG26550.071.4532551
GCGCGTA27250.070.6971051
GCGCTAG33500.069.360011
GTACCGT43300.068.634918
CCGCTAT35850.067.274661
TCCGCGA25050.066.84268
ATAACGC33700.066.330081
GGCGACT37050.064.897271
AACGCGT25250.064.304883
GTTCGCG37000.064.061228
GCCGGAT35300.063.948521
CCGAACG18550.063.692659
CGCGGTA37850.063.3313261
Produced by FastQC (version 0.11.5)