FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19794558
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1883315895.14310953545919No Hit
NAGTAGAG3356221.695526618982854No Hit
AAGGAGAG825130.4168468929692696No Hit
AAGTCGAG567350.2866191808879996No Hit
AAGTGGAG469070.23696917102165152No Hit
ANGTAGAG437370.220954668449783No Hit
AAGTAGCG385790.1948970014889951No Hit
ACGTAGAG379360.19164863393261927RNA PCR Primer, Index 17 (100% over 8bp)
AGGTAGAG375060.18947631970362763No Hit
AAGTTGAG351190.1774174497859462No Hit
AAGAAGAG319610.16146356993674726No Hit
AAGCAGAG292780.1479093395265507No Hit
AAGTAGGG271400.1371083911042621No Hit
AAGTAGAA237760.12011382118256948No Hit
CAGTAGAG235740.11909333868429899No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)