FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005104855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19757494
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13050.080.536969
TTTGCGC32450.079.751811
CGCCGAA31500.079.105481
CGGTACG29750.076.053941
TGCGCGA31750.073.5921
AACGCGT28300.070.7690053
GACGCGT33150.070.484041
CCGCTAT38850.068.1364061
ACGACCG27400.068.004431
ATAACGC38950.067.771641
TCACGCG30250.066.6127248
GTGCGAC31500.066.195091
TAGGCGC38450.065.768361
TTACCCG37500.065.65991
GTACGCT27750.065.300248
CGTTGCG16850.064.957147
GCGCTAG33650.064.382791
TTCCGCG17950.063.399387
CGAACGC37900.063.211051
TCGTACG37950.062.8379258
Produced by FastQC (version 0.11.5)