FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005104857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005104857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19369045
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1828241794.3898731197124No Hit
NAGTAGAG2685991.3867436417231722No Hit
AAGGAGAG1272570.6570122584773798No Hit
AAGTGGAG716280.36980656506296516No Hit
AAGTCGAG629340.3249205110525583No Hit
AGGTAGAG549950.2839324292963334No Hit
AAGTAGGG484430.25010525815805584No Hit
AAGTTGAG478910.24725534996691886No Hit
ACGTAGAG426520.2202070365369072RNA PCR Primer, Index 17 (100% over 8bp)
AAGTAGCG422490.21812639704229092No Hit
AAGAAGAG397700.205327624567964No Hit
AAGCAGAG344300.1777578605450088No Hit
GAGTAGAG321760.16612073543119962No Hit
CAGTAGAG291880.15069405848352357No Hit
AAGTAGAA276310.14265545874874058No Hit
AATTAGAG238090.122922942251412No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)