FastQCFastQC Report
Fri 10 May 2024
EGAF00005105121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005105121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21771539
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1462760.6718679832417911No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG979700.4499911558847539No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG811070.37253682433749863No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA795480.36537609950311734No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG783990.3600985672165849No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG742960.3412528622804295No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA739170.3395120574618083No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA727480.33414266212416127No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT717800.32969649045021576No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT702990.32289403151518137No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT700810.32189272425803245No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG617840.2837833375031503No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA594010.2728378549628485No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT551930.25350986900834155No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG542150.24901776580883878No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC540660.24833338607803518No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG540330.24818181204369613No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT538370.24728155414277328No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT500940.23008938412668023No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT492680.22629544011564823No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA488300.22428363929623898No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC478940.21998444850407683No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA468720.21529024659212195No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT461510.21197858359944147No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT419390.19263222503471158No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG414980.1906066447576352No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA402760.18499381233453455No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT390540.1793809799114339No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA368780.16938627995016797No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT363550.1669840611635218No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT362850.16666254048462076No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA361750.16615729370349058No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC357040.16399391884974232No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT332910.15291064173276864No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT329210.15121117528714897No Hit
GGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGG323100.14840475907559866No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT321900.1478535807689112No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC320890.14738967236078257No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG317200.14569479906771865No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTA307310.14115217119010282No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT301780.1386121578267848No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA300980.13824470562232646No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT284680.13075786695648847No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC278630.12797900966027254No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG275130.12637140626576743No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC273320.1255400456531805No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT264960.1217001701165912No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT258990.11895805804082109No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT256950.11802105491945242No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC256610.11786488773255764No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG251720.11561883613280624No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC249270.11449351375665266No Hit
GCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT248690.1142271109084204No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG242810.11152633720565185No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC242480.11137476317131277No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT240370.11040560798205401No Hit
GCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTT237060.10888527448610776No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA235930.10836624824731039No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG233470.10723633271860111No Hit
GTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGC229950.10561954301898456No Hit
GTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGT229910.10560117040876164No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAG229060.1052107524415247No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT228080.10476062349106326No Hit
AGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTC227090.1043059013880461No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA226900.10421863148948726No Hit
GGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCC221820.10188530999117702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)