FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005105132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005105132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28204232
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG889370.31533211044356746No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA765030.2712465278260369No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT687070.2436052859017753No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA585510.20759650537550534No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT519680.1842560364699879No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG467840.1658758160832034No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGT435810.15451936432802No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC372350.13201919484990762No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC366360.12989540009456735No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTG361670.12823252907577842No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG358430.1270837653016044No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT337730.11974444118882585No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGT329520.11683353051414411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT59500.043.4099431
GGTGGCG287900.037.5643351
GGCGATC75050.034.4825942
GCGATCT82850.031.1415653
TGGGGGG319600.029.9988671
CGATCTG90150.028.6161884
CGGTGGC188650.027.6593041
TGGCGCG397450.026.9186533
GTAGTAC27300.026.20430415
GTGGCGC421600.025.5914692
GGCGCGT482950.021.8986034
CTAGCCT69700.021.679582
GTACGCA20850.021.16440848
GCGACAT124450.020.73236514
TGCGACA125900.020.49373213
TCTATTA79050.019.15339310
GCTGCGA136500.019.00444411
CGCGTGC559350.018.6607976
CTGCGAC140100.018.56564912
GGATAGC50750.018.55417648
Produced by FastQC (version 0.11.5)