FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577280
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT99440.38583312639682144No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT67930.26357244847280853TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGT47510.18434163148745966No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTT42000.1629625031040477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39130.15182673205860442No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT37380.14503662776260245RNA PCR Primer, Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)