FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966550
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT84410.21280457828591598No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA66500.16765198976440482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59170.14917245465202758No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT50690.12779367460387492TruSeq Adapter, Index 11 (95% over 21bp)
GTATAATAACTGGCCTCTCTTCGGCCCAGGGACACGACTGGAGATTAAAC43570.10984356682759576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)