FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699425
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT194680.5262439433155153No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT122370.3307811349061003RNA PCR Primer, Index 17 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGT73600.19894983679896197No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT64430.1741622008825696RNA PCR Primer, Index 17 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTT63260.17099954722693392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.11263912635071666No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)