FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4065907
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT133240.32770055980129403No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT83060.2042840625720165RNA PCR Primer, Index 17 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGT50580.12440028756191422No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT47700.1173169971669298RNA PCR Primer, Index 17 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45790.1126193983285894No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTT45050.11079938621321146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)