FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3453363
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT138160.4000737831499324No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT95100.2753837346378009RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT54860.15885963914016568RNA PCR Primer, Index 17 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGT50250.14551033297107777No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTT49870.1444099563237343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47680.1380683119614127No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)