FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154564
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT113967.372997593229989No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT68704.444760746357495RNA PCR Primer, Index 17 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGT44672.890064956911053No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT36142.338190005434642RNA PCR Primer, Index 17 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTT34252.2159105613208765No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCT17491.1315700939416682RNA PCR Primer, Index 19 (95% over 21bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT14370.9297119639760876RNA PCR Primer, Index 17 (96% over 26bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG13640.882482337413628RNA PCR Primer, Index 17 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC8980.5809891048368314No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG7520.4865298517119122TruSeq Adapter, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCT7420.480060039854041RNA PCR Primer, Index 19 (95% over 24bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCTTG6960.4502989053078337RNA PCR Primer, Index 19 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT6440.41665588364690354No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA4310.2788488910742476RNA PCR Primer, Index 17 (96% over 28bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTG3930.2542636060143371RNA PCR Primer, Index 13 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTC3900.25232266245697577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.22320850909655546No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCT3250.21026888538081312No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC3060.19797624285085788TruSeq Adapter, Index 25 (95% over 24bp)
CTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCTTGA2060.13327812427214616RNA PCR Primer, Index 19 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.11775057581325536No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCG1750.11322170751274553RNA PCR Primer, Index 17 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)