FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385954
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG30500.7902496152391217RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCTTG21320.5523974359638714RNA PCR Primer, Index 26 (96% over 29bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA8180.21194235582478743RNA PCR Primer, Index 26 (100% over 29bp)
CTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCTTGA6030.15623623540629195RNA PCR Primer, Index 26 (96% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT4030.10441658850536592RNA PCR Primer, Index 26 (100% over 27bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC3990.1033801955673474RNA PCR Primer, Index 26 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)