FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103215
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT92840.2991735989933021No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT65520.2111358703795902RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT53920.1737552828276481No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTT52570.16940495582806864RNA PCR Primer, Index 26 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45220.14571984216369152No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT38290.12338816356585026RNA PCR Primer, Index 26 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)