FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559827
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT123700.7930366636812928No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT89400.5731404828868842RNA PCR Primer, Index 26 (100% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTT68080.43645865855636556RNA PCR Primer, Index 26 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT66850.4285731686911433No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT45210.28983983480219283RNA PCR Primer, Index 26 (100% over 24bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCT35540.22784578033333186RNA PCR Primer, Index 26 (95% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG33730.21624192939345196RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28070.1799558540786895No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT23110.1481574559229966RNA PCR Primer, Index 26 (100% over 27bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCTTG22690.14546484962755485RNA PCR Primer, Index 26 (96% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCT17100.10962754202869934RNA PCR Primer, Index 26 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.10219081987938405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)