FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613528
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT91430.566646503810284No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT67730.419763400449202RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT59220.36702183042376707No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTT55420.3434709530916104RNA PCR Primer, Index 26 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT39900.24728421198764444RNA PCR Primer, Index 26 (100% over 24bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCT30370.18822109067831483RNA PCR Primer, Index 26 (95% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG25520.15816273408332548RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21550.13355826487051975No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT19770.12252653812019375RNA PCR Primer, Index 26 (100% over 27bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCTTG17010.10542116405789055RNA PCR Primer, Index 26 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)