FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3657678
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT189100.5169946616405271No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT124650.3407899765917065RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT99500.2720305067859992No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTT89030.2434057891372614RNA PCR Primer, Index 26 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT65870.18008692946727406RNA PCR Primer, Index 26 (100% over 24bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCT45100.12330226991003582RNA PCR Primer, Index 26 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43000.11756092253063283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)