FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429994
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT98930.4071203467992102No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT66910.275350474116397RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT40950.16851893461465337No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTT39340.16189340385202597No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT35760.1471608571873017RNA PCR Primer, Index 27 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35370.14555591495287643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)