FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736849
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT98960.5697674351656362No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT78800.45369516866463344RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT43880.25264142133253953RNA PCR Primer, Index 27 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35950.20698402682098443No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT20650.11889346742290205RNA PCR Primer, Index 27 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTT19890.11451772721750711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.11365409428223179No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT18080.10409655646518494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)