FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549763
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT180750.7088894144279292No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT122210.4792994486154204RNA PCR Primer, Index 27 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTT74350.29159572870105965No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT72870.2857912676589942No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT64890.25449424122947895RNA PCR Primer, Index 27 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTTCT41180.1615052065623354RNA PCR Primer, Index 38 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39690.1556615261889046No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)