FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227482
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT77910.24139561429002546No Hit
TTATTATACTGCTGACAATAATAAACTGCAAAATCTTCAGACTGCAGGCT67630.20954415857315392No Hit
GTATAATAAAGGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA65140.201829165894651No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT54120.16768490110866613RNA PCR Primer, Index 27 (95% over 23bp)
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT44160.1368249303946544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44040.136453123518582No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36490.11306027423235822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)