FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549557
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT159540.625755768551164No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT112850.44262591501190207TruSeq Adapter, Index 1 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTT64390.2525536789332421No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGT61120.23972792136045593No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT59600.23376610132662262RNA PCR Primer, Index 1 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41120.16128292091528057No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTTCT36420.14284834581066436RNA PCR Primer, Index 5 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT31430.12327631819959312TruSeq Adapter, Index 1 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)