FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4252080
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT148410.3490291810125868No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT100730.2368958251020677TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGT56760.1334876107693176No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTT54630.12847829768019417No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT52570.12363360990385881RNA PCR Primer, Index 1 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48090.11309758988542079No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)