FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922716
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT37120.40229062896926027No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT33030.3579649643010417TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT19210.2081897355199216RNA PCR Primer, Index 1 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTT18350.19886942461168983No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGT15900.1723173760940528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15100.1636473194352325No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTTCT11550.12517394301171758RNA PCR Primer, Index 5 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT10380.11249398514819293TruSeq Adapter, Index 1 (96% over 26bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG10080.10924271390113534TruSeq Adapter, Index 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9330.10111453578349133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)